Assessment of Storage-Induced Quality Changes in Freeze-Dried Chicken Liver and Heart Protein Hydrolysates Produced by Lactobacilli Fermentation
Bedika Bora *
Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
Ashim Kumar Biswas
Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
Devendra Kumar
Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
Faslu Rahman CK
Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
Anand TS
Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
Adis Mirel Ahmed
Division of Animal Nutrition, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
The present study evaluated the storage stability of Lactobacillus helveticus (NCDC-292) fermented, freeze-dried chicken liver and heart protein hydrolysates. The resulting chicken liver hydrolysate powder (CLHP) and chicken heart hydrolysate powder (CHHP) were stored aerobically in PET jars at room temperature (27 ± 2°C) and analyzed over a 90-day period at 30-day intervals. Parameters assessed included pH, water activity, lipid oxidation, antioxidant activity, and microbial load. No significant changes in pH were observed throughout the storage period. However, water activity and TBARS values increased significantly (P < 0.05). Lipid oxidation remained within acceptable limits. Antioxidant activity, measured via DPPH and ABTS assays, declined significantly (P < 0.05) over time. A gradual increase in microbial counts was observed after the first 60 days, although the values remained within permissible limits. Overall, significant (P < 0.05) changes were observed in the quality attributes of both hydrolysates during aerobic storage. These findings highlight the necessity for enhanced packaging solutions to maintain the physicochemical and functional integrity of CLHP and CHHP, thereby supporting their potential use as functional food ingredients.
Keywords: Storage stability, hydrolysate, freeze-drying technique, chicken liver, chicken heart, fermentation