Detection of Metallo-beta-lactamase Producing Pseudomonas aeruginosa in an Abidjan Hospital, Côte d’Ivoire
Karine M. K. Gba *
Laboratoire de Génétique, UFR Biosciences, Université Felix Houphouët Boigny, 22BP 582, Abidjan 22, Côte d’Ivoire
Nathalie K. Guessennd
Nathalie K. Guessennd 2Institut Pasteur de Côte d'Ivoire, CHU Cocody-01BP 490 Abidjan 01, Côte d’Ivoire
Nicole P. Nieko Dangui Makaya
Laboratoire de Génétique, UFR Biosciences, Université Felix Houphouët Boigny, 22BP 582, Abidjan 22, Côte d’Ivoire
Eric J. Tahou
Laboratoire de Génétique, UFR Biosciences, Université Felix Houphouët Boigny, 22BP 582, Abidjan 22, Côte d’Ivoire
Fernique Konan
Institut Pasteur de Côte d'Ivoire, CHU Cocody-01BP 490 Abidjan 01, Côte d’Ivoire
Simon-Pierre A. N’guetta
Laboratoire de Génétique, UFR Biosciences, Université Felix Houphouët Boigny, 22BP 582, Abidjan 22, Côte d’Ivoire
*Author to whom correspondence should be addressed.
Abstract
Metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa has emerged as a threat to hospital infection control, due to its ability of multi-drug resistance. This study was carried out to determine the prevalence of MBL producing P. aeruginosa in an Abidjan Hospital, Côte d'Ivoire.
Methods: A prospective study was undertaken to detect MBLs in P. aeruginosa isolates obtained from various clinical samples. A total of 88 strains were screened for imipenem resistance by the disc diffusion method. Detection of MBLs were further done by phenotypic methods, using imipenem-EDTA disk synergy (DST) test and combined disk test (CDT).
Results: Out of 88 isolates, 14 isolates (15.9%) were imipenem resistant. Out 14 imipenem resistant P. aeruginosa strains, 11 were positive for MBL production by CDT and by DST.
Conclusion: Majority (78.6%) of imipenem resistant P. aeruginosa were MBL positive. The prevalence of MBL producing-P. aeruginosa increased from 10.4% in 2011 to 12.5% in 2015 in Abidjan, Côte d'Ivoire. Early detection of carbapenemases and MBL can be performed in hospitals and laboratories from the following rapid and less expensive tests.
Keywords: Metallo-beta-lactamases, Pseudomonas aeruginosa, multidrug resistance