PCR Based Technique for Identification of Bile Salt Hydrolase Positive Lactobacilli
Manjunatha B.M *
Department of Dairy Engineering, College of Dairy Technology, Sri Venkateswara Veterinary University, Tirupati-517502, India.
Spoorthy G.S
Department of Dairy Engineering, College of Dairy Technology, Sri Venkateswara Veterinary University, Tirupati-517502, India.
B. Vijaykumar
Department of Biotechnology, Sri Venkateswara University, Tirupati-517502, India.
*Author to whom correspondence should be addressed.
Abstract
In the burgeoning landscape of probiotic research, the intricate interplay between the gut microbiota and host health has underscored the significance of specific enzymatic activities, notably bile salt hydrolase (BSH) produced by Lactobacilli. Harnessing the potential health benefits associated with BSH activity necessitates a meticulous identification strategy. In response, this study introduces a sophisticated PCR-based technique tailored for the rapid and precise discrimination of lactobacilli strains boasting BSH enzymatic prowess. This PCR-based approach offers a valuable tool for researchers and industry professionals involved in the selection and characterization of probiotic strains with bile salt hydrolase activity. Its speed, accuracy, and adaptability make it a promising method for large-scale screenings and applications in diverse fields, ranging from food science to the development of probiotic supplements aimed at enhancing human health through the modulation of bile acid metabolism. robiotic supplementation, where the modulation of bile acid metabolism holds promise for promoting human health and well-being.
Keywords: Bile salt hydrolase, positive lactobacilli strains, PCR