Antimicrobial Resistance Profile and Molecular Characterization of Extended-spectrum Beta-lactamase Genes in Enterobacteria Isolated from Human, Animal and Environment
Gbonon M’bengue Valérie Carole *
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Guessennd Nathalie Kouadio
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet - Boigny University, Abidjan, Côte d'Ivoire
Ouattara Mohamed Baguy
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire
Ouattara Gnoh Djénéba
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Abraham Ayayi
Department of Microbiology, University of Lagos, Nigeria
Tiekoura Konan Bertin
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Toty Abalé Anatole
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Kouadio Kouamé Innocent
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet - Boigny University, Abidjan, Côte d'Ivoire
Dissinviel Stéphane Kpoda
Laboratory of Applied and Nutritional Sciences, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
Tahou Eric
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Konate Ali
Laboratory of Molecular Biology, Epidemiology and Monitoring of Foodborne Bacteria and Viruses, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
Konan Fernique
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Kamenan Alphonse
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire
Dosso Mireille
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
Ger Bmr
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
*Author to whom correspondence should be addressed.
Abstract
Objective: The aim of this study is to determine the antibiotic resistance profile and characterize extended spectrum beta-lactamase gene of enterobacteria strains isolated from human biological products, fecal matter of animals and the environment.
Materials and Methods: Enterobacteria producing ESBL strains were isolated from human products, fecal matter of healthy animals (cattle, sheep and pigs) intended for human consumption and environment (hospital effluents and municipal sewage) using homemade medium (Drigalski supplemented with 2 mg/L of ceftazidime). Resistance to beta-lactams has been evaluated by the diffusion method was carried out as recommended by NCCLS. Characterization of Beta-Lactamase resistance genes (blaCTXM, blaSHV, blaTEM, blaGES, blaPER and blaVEB) was performed by simplex and multiplex PCR.
Results: The strains were resistant to antibiotics from beta-lactam family (penicillin with inhibitor, monobactam, cephalosporin) but no resistant was observed to carbapenem (imipénème, méropénème). All resistance genes were identified in environment strains.
Conclusion: This study showed the presence of common beta-lactam resistance genes (blaTEM, blaSHV and blaCTX-M) to human, animal and environment. The risk of dissemination and circulation of ESBL enterobacteria between animals, humans and the environment exists in Ivory Coast because of the absence of a barrier between them.
Keywords: Enterobacteria ESBL, human, humananimal, environment, Ivory Coast