Screening of in vitro Antibacterial Activity of Rumex vesicarius (L.) Leaves Extract against Twelve Pathogenic Bacterial
Arnaba Saha Chaity
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Md. Ashikul Islam
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Tamanna Nasrin
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Sathi Rani Sarker
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Amit Kumar Dutta
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Biswanath Sikdar
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Md. Faruk Hasan *
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
There is an alternative approaches from eradication of infections causes by pathogenic bacteria especially resistant bacteria. Methanol extract of Rumex vesicarius leaves were evaluated from in vitro antibacterial activity against twelve bacterial species were used which are four of them gram positive which are Streptococcus constellatus, Staphylococcus gallinarum, Staphylococcus sciuri and Streptococcus iniae and eight of them gram negative which are Aeromonas diversa, Xanthomonas campestris, Xanthomonas axonopodies, Siccibacter colletis, Edwardsielloa anguillarum, Aeromonas cavernicala, Enterobacter xiangfangenis and Vibro rotiferianus. The plant extract showed highest 12 mm zone of inhibition against Staphylococcus constellatus at the concentration of 20 μg/disc and no zone of inhibition was found from Aeromonas diversa. In minimum inhibitory concentration (MIC) test, methanol extract of Rumex vesicarious in 200μg/ml concentration showed best result against Vibrio rotiferianus. It can be concluded that methanol extracts of Rumex vesicarious leaves may be used as natural antibacterial from treatment of some diseases, especially local skin diseases.
Keywords: Rumex vesicarius extract, bacteria, MIC values.