Competitiveness among Rhizobia for Nodulation of Introduced Leguminous Tree Gliricidia sepium in a Sub-Saharan Sandy Soil
Adama Diouf *
Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P 5005, Dakar, Sénégal and Laboratoire Commun de Microbiologie IRD/ISRA/UCAD, Centre de recherche de Bel Air, B.P. 1386, Dakar, Sénégal.
Malick Ndiaye
Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P 5005, Dakar, Sénégal.
Mame Arama Fall-Ndiaye
Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P 5005, Dakar, Sénégal.
Tahir Abdoulaye Diop
Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P 5005, Dakar, Sénégal.
*Author to whom correspondence should be addressed.
Abstract
Agroforestry systems are progressively integrated by small farmer holder to mitigate agricultural production charge and contribute to sustainable agriculture by restoring and maintaining sandy soil fertility. A greenhouse experiment was carried out to test the nodulation level of introduced Gliricidia sepium tree with foreign rhizobial reference strains TAL1769 and TAL1770 against native rhizobial community using PCR/RFLP techniques. Restriction patterns of the two inoculated reference strains obtained for 16S – 23S rDNA - IGS were different to those of indigenous rhizobia detected in all remained nodules collected from root plants regarding the restriction enzymes HaeIII and MspI. Nodule occupancy rates of the reference strains and native rhizobial strains of profile F ranged from 18.36 to 22.45 and were higher compared to others rhizobial strains detected in gliricidia rhizosphere. Therefore, regarding its high competitiveness level, the native rhizobial strain may be considered as a good candidate to inoculate the introduced legume tree gliricidia.
Keywords: Competitiveness, gliricidia, nodule occupancy, rhizobial strains