In vitro Evaluation of Spectrum of Antimicrobial Activities of Leaves and Stem Bark Extracts of Alchornea cordifolia against Some Pathogenic Clinical Isolates from University of Uyo Medical Centre

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A. A. Agboke
I. F. Ubak
C. N. Nwosu
M. H. Ekanem
M. A. Momoh


Antimicrobial activities of the leaves and stem bark in aqueouse and ethanol extracts of Alchornea cordifolia were evaluated using tube dilution assay to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) of the plant parts and to determine spectrum of activities considering the antimicrobial effect on Gram positive, Gram negative and yeast (Candida albicans), these organisms are known to be pathogenic in nature. Results showed leaves extracts, both aqueouse and ethanol exerted higher activities than the stem bark extracts with MIC values ranging from 1.95 mg/mL – 15.63 mg/mL, although the stem bark extract exhibited good inhibitory activities as well with MIC values ranging from 3.91 mg/mL – 62.50 mg/mL. The antimicrobial spectrum of activities against the organisms was determined by the ratio (R) of MBC/MIC. The leaf extract is bactericidal against the Gram-positive and Gram negative organism with the exception of E. coli that MBC was not determined and, fungicidal against Candida albicans with (R) MBC/MIC ≤ 2. The stem bark extracts were bactericidal against all the Gram positive organisms but bacteriostatic against Salmonella spp and fungistatic against Candida albicans with (R) MBC/MIC ≥ 2. This study revealed that the aqueous and ethanol extracts of leaf and stem bark of A. cordifolia plant exhibited strong antibacterial activities on Staphylococcus aureus, Streptococcus pneumoniae, Bacillus Subtilis, (Gram positive), Escherichia coli, Pseudomonas aeruginosa, Salmonella spp (Gram negative) and also exhibit anti-fungal activity against the Candida albicans. The leaf extract exhibited broad antimicrobial spectrum with bactericidal activities against Gram positive, Gram negative and yeast (Candida albicans).

Alchornea cordifolia, aquoues, ehanol extracts, bacteriostatic, bactericidal, fungicidal activities.

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Agboke, A. A., Ubak, I. F., Nwosu, C. N., Ekanem, M. H., & Momoh, M. A. (2020). In vitro Evaluation of Spectrum of Antimicrobial Activities of Leaves and Stem Bark Extracts of Alchornea cordifolia against Some Pathogenic Clinical Isolates from University of Uyo Medical Centre. Journal of Advances in Microbiology, 20(4), 58-65.
Original Research Article


Joseph N, Sorel NEM, Félicien MK, Emmanuel MM. Phytochemical screening and antibacterial properties from extract of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. J. Pharmacogn. Phytochem. 2015;4(3):176–180.

Ebenyi LN, Nwakaeze AE, Moses IB, Iroha IR, Uzoeto JO, Ugochukwu JI, Eddison IO, Okamkpa CJ. Antibacterial activity of Alchornea cordifolia leaves found in Ebonyi state, Nigeria. International Journal of Advances In Pharmacy, Biology and Chemistry. 2017;6(1):46.
[ISSN: 2277 – 4688]

Mavar-Manga H, Brkic D, Marie DEP, Quetin-Leclercq J. In-vivo anti-inflammatory activity of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. (Euphorbiaceae). J Ethnopharmacol. 2004; 92:209–214.

Ajibade TO, Olayemi FO. Reproductive and toxic effects of methanol extract of Alchornea cordifolia leaf in male rats. Andrologia. 2015;47(9):1034–1040.

Chinsembu KC. Plants as antimalarial agents in Sub-Saharan Africa. Acta Trop. 2015;152:32–48.

Osadebe PO, Okoye FBC, Uzor PF, Nnamani NR, Adiele IE, Obiano NC. Phytochemical analysis, hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac J Trop Med. 2012;5:289-293.

Boniface PK, Ferreira SB, Kaiser CR. Recent trends in phytochemistry, etnobotany and pharmacological significance of Alchornea cordifolia (Schumach & Thonn.) Muell. Arg. Journal of Ethnopharmacology. 2016;191:216-244.

Ayisi NK, Nyadedzor C. Comparative in vitro effects of AZT and extracts of Ocimumgratissimum, Ficuspolita, Clausenaanisata, Alchornea cordifolia, and Elaeophorbiadrupifera against HIV-1 and HIV-2 infections. Antivir. Res. 2003;58(1): 25–33.

Siwe-Noundou X, Musyoka TM, Moses V, Ndinteh DT, Mnkandhla D, Hoppe H, Bishop ÖT, Krause RWM. Anti-HIV-1 integrase potency of methylgallate from Alchornea cordifolia using in vitro and in silico approaches. Scientific Reports. 2019; 9:4718.

Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 2015;4:196.

Sudhakar M, Rau CV, Roa PM, Rajo DB. Evaluation of antimicrobial activity of Cleome Viscose and G. Asiatic. Fitoterapia. 2006;77:47-49.

Gatsing D, Nkeugouapi CFN, Nji-Nkah BF, Kuiate JR, Tchouanguep FM. Antibacterial activity, bioavailability and acute toxicity evaluation the leaf extract of Alchornea cordifolia (Euphorbiaceae). Int J Pharmacol. 2010;6:173–182.

Pesewu AG, Ronald RC, David PH, Winston AM. Evaluation of antibacterial activity and phytoconstituents of the aqueous leaves extract of Alchornea cordifolia, Int. Sci. Res. J. 2015;1 (7):48–54.

Adeshina GO, Kunle OF, Onaolapo JA, Ehinmidu JO, Odama LE. Antimicrobial activity of the aqueous and ethyl acetate sub-fractions of Alchornea cordifolia leaf. European Journal of Medicinal Plants. 2012;2(1):31-41.

Siwe-Noundou X, Krause RW, Van-Vuuren SF, Ntinteh DT, Olivier DK. Antibacterial seffects of Alchornea cordfolia (Schumach. & Thonn.) Müll. Arg. extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. J. Ethnopharmacol. 2016;179:76–82.

Ebessa V, Tiani GLM, Tchamgoue J, Kouam SF, Ngadjui BT, Green IR, Choudhary MI, Tane P. Alchornoic acid derivatives from the fruits of Alchornea cordifolia (Schumach. & Thonn.) Muell. Arg. (Euphorbiaceae). Phytochemistry Letters. 2018;23:62–65.

CimangaKanyanga R, Makila Bool-Miting F, Tona Lutete G, Kambu Kabangu O, Vlietinck AJ, Pieters L. Antibacterial screening of aqueous extracts of some medicinal plants and their fractions used as antidiarrheal agents In Kinshasa-Democratic Republic of Congo. World Journal of Pharmacy and Pharmaceutical Sciences. 2018;7(1):223-242.

Geo F. Brooks, Karen C. Carroll, Janet S. Butel, Stephen A. Morse, Timothy A. Mietzner. Jawetz, Melnick and Adelberg’s Medical Microbiology. 26th edition, Prentice-Hall, New York. 2013;63.