Genomic characterization and Phylogenetic analysis of Tomato leaf curl virus (ToLCV) from Bangladesh
M. M. Hasan *
Advanced Seed Research and Biotech Centre, Dhaka, Bangladesh.
M. B. Meah
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Y. Sano
Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.
*Author to whom correspondence should be addressed.
Abstract
Tomato leaf curl virus (ToLCV) has frequently emerged as a severe problem for tomato in the recent past, in the tropical and subtropical region of the world. We have cloned and sequenced two isolates of ToLCV responsible for the leaf curl disease of tomato in Bangladesh. Two betasatellite DNAs were associated with ToLCV, and complete nucleotide sequences were determined. The complete genome sequences of ToLCV-[BD:Mym:11] and ToLCV-[BD:Mym2:11] shared the highest nucleotide sequence identities at 95.04 and 92.38%, respectively, with Indian isolate of Tomato leaf curl virus - [India:Ranchi:2007] (ToLCV-[IN:Ran:07]) and Tomato leaf curl Patna virus - [India:Lucknow:2009] (ToLCPatV-[IN:PLuc:09]). Complete nucleotide sequence analysis of the two betasatellite clones, tomato leaf curl betasatellite – [BD:Mym:11] and tomato leaf curl betasatellite – [BD:Mym2:11] associated with (ToLCV-[BD:Mym:11] and (ToLCV-[BD:Mym2:11], when used in BLAST searches respectively revealed 95.51 and 86.43% identity with Tomato leaf curl Bangladesh betasatellite (ToLCBDB-[BD-Gaz-01]) and Tomato leaf curl Patna betasatellite (ToLCPaB-[IN-Pat-07]).
Keywords: Betasatellite, genomic characterization, phytogenetic tree, ToLCV