Antibacterial Activity of Honey on Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes Isolated from Wounds

Main Article Content

V. U. Usanga
M. O. Elom
N. O. Umoh
A. Nworie
B. N. Ukwah
M. E. Kalu
S. O. Azi

Abstract

Aim: To assess the antibacterial activity of crude honey obtained from Ishielu Local Government of Ebonyi State on pathogenic bacterial species (Staphylococcus aureus, Escherichia coli and Staphylococcus pyogenes) isolated from wounds.

Study Design: An experimental study which involved a random selection of patients with wound

Place and Duration of Study: Department of Medical Laboratory Science, Ebonyi State University, Abakaliki, Nigeria, between February 2019 and November 2019.

Methodology: A total of 50 samples of wound swabs collected from different sites of open wounds were cultured on blood agar, chocolate agar and MacConkey agar. The crude honey was diluted to concentrations ranging from 20% to 100% and the antibacterial activity was carried out by well diffusion method with augmentin used as a control.

Result: Out of the 50 samples, 43 showed growth of bacterial species isolated, identified and confirmed using standard bacteriological techniques. Staphylococcus aureus (60.5%) was the most frequent isolates, followed by Escherichia coli (27.9%) and Streptococcus pyogenes (11.6%). All the tested bacterial isolates were susceptible to the honey and the number of isolates as well as the diameter of zone of inhibition was positively linearly correlated with increasing concentration of the honey (p= 0.00). At 100% honey, 22 (22.25±0.46 mm) out of 26 Staphylococcus aureus were susceptible as against 8 (4.62±0.31 mm) at 20% honey.  The number of Escherichia coli inhibited at 20% honey was 2 (1.96±0.04 mm) out of 12 isolated and at 100%, 7 (19.17±0.31 mm) were inhibited. At 20% honey, no Streptococcus pyogenes was inhibited and at 100%, 4 (21.84±0.15 mm) out of 5 isolated were inhibited.

Conclusion: Locally produced crude honey may be used as a source of an effective antibacterial agent for wound management.

Keywords:
Antibacterial activity, honey, Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, wounds

Article Details

How to Cite
Usanga, V. U., Elom, M. O., Umoh, N. O., Nworie, A., Ukwah, B. N., Kalu, M. E., & Azi, S. O. (2020). Antibacterial Activity of Honey on Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes Isolated from Wounds. Journal of Advances in Microbiology, 20(4), 51-57. https://doi.org/10.9734/jamb/2020/v20i430236
Section
Original Research Article

References

Bowler HD. Signs of clinical infection by stable poly-microbial communities. Clinical Wound Management. 2011;84(3):179.

Hanson T, Van Den Bogard AE, Hazan IN. Honey for wounds, ulcers and skin graft preservation. Lancet. 1995;341:756-757.

Emmerson E. Presence of pathogens in wounds and the delicate balance between a colonized wound and an infected wound. Journal of Science. 1998;73(1):5-28.

Panghal M, Singha K, Kadyana S, Chaudary U, Yadav JP. The analysis of distribution of multidrug resistant Pseudomonas and Bacillus species from burn patients and burn ward environment. Burns. 2015;41:812-819.

Ghosh Dastidar M, Razia M. Isolation of multiple drug resistant (MDR) bacteria from hospital environment. International Journal of Current Microbiology and Applied Sciences. 2016;5:48-53.

Anguzu JR, Olila D. Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda. African Health Science. 2007;7(3):148-154.

Bereket W, Hemalatha K, Getenet B, Wondwossen T, Solomon A, Zeynudin A, et al. Update on bacterial nosocomial infection. European Review for Medical and Pharmacological Sciences. 2012; 16(8):1039-1044.

Shittu AO, Kolawole DO, Oyedepo EAR. A study of wound infections in two health institutions in Ile-Ife, Nigeria, African Journal of Biomedical Research. 2002;5: 97-102

Rossiter SE, Fletcher MH, Wuest WM. Natural products as platforms to overcome antibiotic resistance. Chemical Reviews. 2017;117(19):12415-12474.
DOI: 10.1021/acs.chemrev.7b00283

Gills IS. Ethno-medical uses of plants in Nigeria. Illupeju Press Ltd. 1992;165-250.

Moloney MG. Natural products as a source for novel antibiotics. Trends in Pharmaco-logical Sciences. 2016;37(8):689-701.

Carter DA, Blair SE, Cokcetin NN, Bouzo D, Brooks P, Schothauer R, et al. Therapeutic manuka honey: No longer so alternative. Frontiers in Microbiology. 2016; 7:569.
DOI: 10.3389/fmicb.2016.00569

Gunther RT. Antibacterial activity of honey on rotten wounds and hollow ulcer. Wound Care. 1958;13:300-351.

Bogdanov S, Haldimann M, Luginbuhl W, Gallmann P. Minerals in honey: environmental, geographical and botanical microbiology. 2007;3(1):398-400.

Molan PC, Cooper RA. Honey and sugar as dressing for wounds and ulcers. Tropical Doctor. 2000;30:249-251.

Alnaimat S, Wainwright M, AľAbriK. Antibacterial potential of honey from different origins: A comparison with Manuka honey. Journal of Microbiology, Biotechnology and Food Sciences. 2012; 3(1):398-400.

Velikova M, Bankova V, Tsvatkova I, Kujumgiev A, Marcucci MC. Antibacterial Entkaurene from Brazilian propolis of native stingless bees. Fitoterapia. 2000;71: 693-696.

Marcucci MC, Bankova VS. Chemical composition, plant origin and biological activity of Brazilian propolis. Current Topics inPhytochemistry. 2001;2:115-123.

Cheesbrough M. Medical laboratory manual for tropical countries. Cambridge: Cambridge University Press; 2010.

Aynakem M, Mengistu ES, Teklay G, Moges T, Feleke M. Bacterial isolates and their antimicrobial susceptibility patterns of wound infections among inpatients and outpatients attending the University of Gondar Referral Hospital, Northwest Ethiopia. International Journal of Microbiology; 2017.
DOI: doi.org/10.1155/2017/8953829

Surajit R, Manisha M, Nishith KP, Malay KD, Debashis H, Bijayanta S, et al. Exploration of antibacterial and antioxidative property of two natural honey samples from Malda District, India. Translational Medicine. 2016;6:4.
DOI: 10.4172/2161-1025.1000187

Okeke O, Okeke MU, Ezejiofor CC, Ndubuisi JO. Antimicrobial activity of honeys from Nsukka and Ugwuji in Enugu State, on selected pathogenic bacterial isolated from wound. Advances in Analytical Chemistry. 2018;8(1):6-9.

Farrag HA, El-Rehim AH, Hazzan MM, El-Bayed AB. Prevalence of pathogenic bacterial isolates infecting wounds and their antibiotic sensitivity. Journal of Infectious Diseases and Treatment. 2016; 4:5.
DOI: 10.4172/2332-0877.1000300

Kibret M, Abera B. Bacteriology and antibiogram of pathogens from wound infections at Dessie Laboratory, North East Ethiopia. Tanzania Journal of Health Research. 2011;13(4):1-11.

Garba I, Lusa Y, Bawa E, Tijjani M, Aliyu N, Raji U. Antibiotics susceptibility pattern of Pseudomonas aeruginosa isolated from wounds in patients attending Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. Nigerian Journal of Basic and Applied Science. 2012;20:32- 34.

Giacometti A, Cirioni O, Schimizzi AM, Del Prete MS, Barchiesi F, D’Errico MM, et al. Epidemiology and microbiology of surgical wound infections. Journal of Clinical Microbiology. 2000;38(2):918-922.

Ohalete CN, Obi RK, Emea Koroha MC. Bacteriology of different wound infection and their antimicrobial susceptibility patterns in Imo state Nigeria. World Journal of Pharmaceutical Sciences. 2012; 13(3):1155-1172.

Reham FE. Natural antibacterial remedy for respiratory tract infections. Asian Pacific Journal of Tropical Biomedicine. 2016;6(3):270-274.

Mandal MD, Mandal S. Honey: Its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine. 2011;1(2):154-160.

Tin SS, Wiwanitkit V. Honey dressing vs. povidone iodine dressing. Indian Journal of Surgery; 2015.
DOI: doi.org/10.1007/s12262-014-1198-z

Ali M. Hydrogen peroxide therapies: Recent insights into oxystatic and antimicrobial actions. Townsend Letter for Doctors and Patients. 2004;255:140.

White Jr. JW, Subers MH, Schepartz AI. The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochimica et Biophysica Acta. 1963;73(1):57-70.

Brudzynski K. Effect of hydrogen peroxide on antibacterial activities of Canadian honeys. Canadian Journal of Microbiology. 2006;52(12):1228-1237.

Wahdan HAI. Causes of the antimicrobial activity of honey. Infection. 1998;26(1):26-31.

Eteraf-Oskouei T, Najafi M. Traditional and modern uses of natural honey in human diseases: A review. Iranian Journal of Basic Medical Sciences. 2013;16:731-742.

Almasaudi SB, Alea AM, Elsajed M, Yousef AQ, Esan A, Mohamad Q. Antimicrobial effect of different types of honey on Staphylococcus aureus. Saudi Journal of Biological Science. 2017;24: 1255-1261.