Growth Rate and Antifungal Activities of Acetone Extracts of Ocimum gratissimum (Scent Leaf) and Allium sativum (Garlic) on Cassava and Banana Peels Formulated Media

Main Article Content

Ebele Linda Okoye
Bright Obidinma Uba
Uzoma Collins Dike
Chimezie Justin Eziefule

Abstract

Aim: To study the growth rate and antifungal activities of acetone extracts of Ocimum gratissimum (scent leaf) and Allium sativum (garlic) on cassava and banana peels formulated media.

Study Design: Nine treatments and control designs were set up in triplicates and incubated at 25  for 72 h. The nine treatments and control set ups designated as Cassava Glucose Agar, CGA), Banana Glucose Agar, (BGA) and Control (Potato Dextrose Agar, PDA) were used to screen for the growth rate and antifungal activities of plant extracts.

Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Nigeria between June, 2019 and August, 2019.

Methodology: The research study was carried on two formulated media obtained from cassava and banana peels and PDA) using proximate analysis, pour plate technique, agar plug diffusion technique and agar well diffusion technique.

Results: The results revealed that the cassava and banana peels contained nutrients that can meet the nutritional conditions for fungi cultivation. The PDA statistically (P < 0.05) had higher mean radial growth (32.33 mm) and growth rate (0.449 h-1) than BGA (14.33 mm; 0.199 h-1) followed by CGA (14.16 mm; 0.197 h-1) in most of the test fungal isolates. The scent leaf acetone extract and nystatin antifungal had higher zones of inhibition (25.00 mm) than garlic acetone extract (24.00 mm) on the tested fungal isolates. There was no statistical significance at P < 0.05 on the inhibition zones of the extracts and Nystatin on the formulated media and PDA showing that the extracts possess comparable antifungal activities to the Nystatin.

Conclusion: Thus, our formulated media were comparable to PDA with regards to antifungal activity of the acetone extracts and nystatin and could be utilized as an alternative and cheap ideal reference media for mycological assays.

Keywords:
Cassava glucose agar, banana glucose agar, extracts, susceptibility testing, antifungal activity.

Article Details

How to Cite
Okoye, E. L., Uba, B. O., Dike, U. C., & Eziefule, C. J. (2020). Growth Rate and Antifungal Activities of Acetone Extracts of Ocimum gratissimum (Scent Leaf) and Allium sativum (Garlic) on Cassava and Banana Peels Formulated Media. Journal of Advances in Microbiology, 20(4), 19-29. https://doi.org/10.9734/jamb/2020/v20i430233
Section
Original Research Article

References

Nwabueze T, Otuwa U. Effect of supplementation of African bread fruit (Treculia africana) hulls with organic wastes on growth of Saccharomyces cerevisiae. Afr J Biotech. 2006;5(16):1494 – 1498.

Amadi OC, Moneke AN. Use of starch containing tubers for the formulation of culture media for fungal cultivation. Afr J Microbiol Res. 2012;6(21):4527–4532

ItelimaJ, Onyimba I, Nyam M, Nwachukwu I. Utilization of food crop wastes for the formulation of laboratory media used for cultivating soil fungi. Int J Microbiol Imm Res. 2014;2(1):10–14.

Kuhn DM, Ghonnoum MA. Indoor mold, toxigenic fungi and Stachybotrys chartarum: Infectious disease perspective. Clin. Microbiol. Rev. 2003;16(1):144– 172.

Kumara KLW, Rawal RD. Influence of carbon, nitrogen, temperature and pH on the growth and sporulation of some Indian isolates of Colletotrichum gloeosporioides causing anthracnose disease of papaya (Carrica papaya L). Trop. Agric. Res. Ext. 11:7–12.

Akharaiyi FC, Abiola MA. Isolation and cultivation of fungi with agrowastes formulated media. Der Pharm Chem. 2016; 8(9):56–62.

Anbu S, Padma J, Punithavalli K, Saranraj P. Fruits peel waste as a novel media for the growth of economically important fungi. J Pharmacog Phytochem. 2017;6(6):426– 428.

AOAC. Official methods of analysis of the association of official analytical chemistry. 16th edition. Washington, USA: AOAC International; 2000.

Onome F, Ejale AU. An attempt to formulate culture media for the culture of air-borne fungi using local plant flours. Iss Biol Sci Pharm Res. 2018;6(3):39–45.

Sah SN, Ghimire T, Yadav SP, Shah PK. Growth pattern of economically important fungi in fruits peel waste media. Int J Grad Res Rev. 2019;5(1):60–66.

Uba BO, Okoye EL, Anyaeji OJ, Ogbonnaya OC. Potentials of actinomycetes isolated from coastal area of Niger Delta against Citrus sinensis (Sweet Orange) and Lycopersicum esculentum (Tomato) fungal pathogens. ResRev: A JBiotech. 2019;8(3):4–15.

Adesemoye AO, Adedire CO. Use of cereals as basal medium for the formulation of alternative culture medium for fungi. W Afr J Microbiol Biotech. 2005; 21:329–336.

Laleye SA, Tedela PO, Adesua B, Famurewa O. Growth of some microorganisms on media formulated from local raw materials. Res J Microbiol. 2007; 2:545–549.

Umedum CU, Enejekwute NP. Exploration of fungi growth on media formulated from agro-allied wastes. Trop J Appl Nat Sci. 2017;2(1):69–73.

Prescot LM, Harley DA. Microbiology. 5th edition. London: McGraw-Hill Publishers; 2002.

Ruth AO, Gabriel A, Mirrila EB. Basal media formulation using Canavalia ensiformis as carbon and nitrogen source for the growth of some fungi species. J Microbiol Biotech F Sci. 2012;1(4):1136–1151.

Uba BO, Okoye EL, Udeajah OP. Antimicrobial activities of A. sativum, Z. officinale and O. gratissimum extracts on plant and fish pathogens. Afr J Edu Sci Tech. 2016;3(2):213–221.