Effect of Heavy Metals on β-galactosidase Activity in Marine Bacteria

Main Article Content

Ogechi Joy Osigwe
Caroline Nchedo Ariole
Abiye Anthony Ibiene

Abstract

Background: Due to metal pollution and its toxicity in the coastal areas, the enzymatic activities of bacteria involved in the breakdown of organic compounds are repressed leading to decline in biodegradation rate.

Aim: The influence of heavy metals (copper, lead, zinc, manganese and iron) on β-galactosidase activity in three bacterial strains (Providencia stuartii, Pantoea dispersa and Aeromonas dhakensis) isolated from coastal marine sediment collected from coastal zone in Bonny Island, Nigeria was investigated.

Methodology: The strains were cultivated in Z- buffered medium having lactose as enzyme inducer. Beta galactosidase assay was done via 2-nitrophenol β-D-galactopyranoside as the substrate. The absorbances of p-nitrophenol solution formed were measured at 420 nm in a spectrophotometer. The β-galactosidase activities were calculated comparative to controls.

Results: Presence of the metals significantly affected β-galactosidase activities. Metal concentration of 0.001 mg/L triggered a decrease in enzyme activity. The sensitivity patterns of Pantoea dispersa and Aeromonas dhakensis were Cu>Pb>Zn>Mn>Fe while that of Providencia stuartii was Pb>Cu>Zn>Mn>Fe. The effect of metal stress to enzyme synthesis is reliant on the organism and the metal. This might be described with logistic dose-response model using elevated coefficient of inhibition (R > 0.81).

Conclusion: The results revealed that concentration of metal as low as 0.001 mg/L when deposited in the environment has detrimental effect on microbial activities and consequently on biogeochemical cycles. The isolated bacterial strains could serve as ideal organisms for heavy metal toxicity evaluation.

Keywords:
Enzyme activity, β-galactosidase, heavy metals, pollution, sediment.

Article Details

How to Cite
Joy Osigwe, O., Nchedo Ariole, C., & Anthony Ibiene, A. (2020). Effect of Heavy Metals on β-galactosidase Activity in Marine Bacteria. Journal of Advances in Microbiology, 20(1), 32-43. https://doi.org/10.9734/jamb/2020/v20i130207
Section
Original Research Article

References

Fergusson JE. The heavy elements: chemistry, environmental impact and health effects. Pergamon Press, Oxford; 1990.

Arruti A, Fernández-Olmo I, Irabien A. Evaluation of the contribution of local sources to trace metals levels in urban PM2.5 and PM10 in the Cantabria region (Northern Spain). Journal of Environmental Monitoring. 2010;12(7):1451–1458.

Pacyna JM. Monitoring and assessment of metal contaminants in the air. Toxicology of Metals. 1996;9–28.

WHO/FAO/IAEA. Trace elements in human nutrition and health. World Health Organization. Switzerland: Geneva; 1996.

Perra G, Renzi M, Guerranti C, Focardi SE. Polycyclic aromatic hydrocarbons pollution in sediments: Distribution and sources in a lagoon system (Orbetello, Central Italy). Transit Wat Bull. 2009;3: 45–58.

Da Silva TF, De Azevedo DA, De Aquino Neto FR. Distribution of polycyclic aromatic hydrocarbons in surface sediments and waters from Guanabara Bay, Rio de Janeiro, Brazil. J Braz Chem Soc. 2007; 18:628–637.

Barbier EB, Hacker SD, Kennedy C, Koch EW, Stier AC, Silliman BR. The value of estuarine and coastal ecosystem services. Ecol Monogr. 2011;81:169-193.

Cabello P, Roldan MD, Moreno-Vivian C. Nitrate reduction and the nitrogen cycle in archaea. Microbiology. 2004;150(11): 3527-3546.

Schlesinger W. Biogeochemistry; an analysis of global change. San Diego Academic; 1997.

Nweke CO, Okpokwasili GC. Inhibition of β-galactosidase and α-glucosidase synthesis in petroleum refinery effluent bacteria by zinc and cadmium. Journal of Environmental Chemistry and Ecotoxico-logy. 2011;3(3):68-74.

Saitou N, Nei M. The Neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4: 406-425.

Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985;39:783-791.

Jukes TH, Cantor CR. Evolution of protein molecules. In: Munro HN. (ed.) Mammalian Protein Metabolism, Academic Press, New York. 1969;21-132.

Kurmasheva N, Vorobiev V, Sharipova M, Efremova T, Mardanova A. The potential virulence factors of Providencia stuartii: motility, adherence and invasion. BioMed Research International. 2018;1-8.

Mehar V, Yadav D, Sanghvi J, Gupta N, Singh K. Pantoea dispersa: An unusual cause of neonatal sepsis. The Brazilian Journal of Infectious Diseases. 2013; 17(6):726-728.

Melo-Bolivar JF, Sinclair HA, Sidjabat HE. Microbiology resource announcements. Draft genome sequence of Aeromonas dhakensis isolated from a patient with fatal necrotizing fasciitis. Microbiol Resour Announc. 2019;8(22):1-2.

Ariole CN, Onwudiwe EON, Okpokwasili GSC. Diversity and antibacterial potential of culturable coastal marine bacteria. J Glob Eco Environ. 2017;6(4): 149-159.

Kumar S, Chaudhuri S, Maiti SK. Soil dehydrogenase enzyme activity in natural and mine soil – A review. Middle-Eat Journal of Scientific Research. 2013; 13(7):898–906.

Pepper LL, Gerba CP, Gentry TJ. Environmental microbiology. Third Edition. Academic Press. 2015;111–136.

Kharchenko UV, Beleneva IA, Kovalchuk Yu l, Hiep LTM. Enzymatic indication of heavy metal toxicity to marine heterotrophic bacteria. Russian Journal of Marine Biology. 2013;39(4):287-294.

Dutton RJ, Bitton G, Koopman B. Enzyme biosynthesis versus enzyme activity as a basis for microbial toxicity testing. Journal of Toxicology. 1988;3:245–253.

Pan J, Yu L. Effects of Cd or/and Pb on soil enzyme activities and microbial community structure. Ecological Engineer-ing. 2011;37:1889-1894.

Odokuma LO, Akponah E, Effect of concentration and contact time on heavy metal uptake by three bacterial isolates. Journal of Environmental Chemistry and Ecotoxicology. 2010;2(6):84-97.

Nweke CO, Okolo JC, Nwanyanwu CE, Alisi CS. Response to planktonic bacteria of new Calabar River to zinc stress. Afri. J. Biotechnol. 2006;5(8):653-658.

Rensing C, Grass G. Escherichia coli mechanisms of copper homeostasis in a changing environment. FEMS Microbiol. Rev. 2003;27:197–213.

Santo CE, Lam EW, Elowsky CG, Quaranta D, Domaille DW, Chang CJ, Grass G. Bacterial killing by dry metallic copper surfaces. Applied and Environ-mental Microbiology. 2011;77(3):794- 802.

Kalantari N, Ghaffari S. Evaluation of Toxicity of heavy metals for Escherichia coli growth. Iranian Journal of Environmental Health Science & Engineering. 2008;5(3):173-178.